999久久久国产精品,国产情侣一区二区,亚洲AV中文无码乱人伦在线视色,国产欧美一区二区三区在线看,欧美一区精品视频一区二区,色老头在线一区二区三区,又大又粗又硬又爽又黄毛片,国产98在线免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pDMS197
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pDMS197
pDMS197
規(guī)格:
貨期:
編號(hào):B161742
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDMS197
商品貨號(hào) B161742
Designations pDMS197
Depositors DM Schifferli
Biosafety Level 1
Host Escherichia coli SM10 lambda pir
Vector Information
Size (kb): 6.50
Type of vector: plasmid
Cloning sites: BamHI XbaI KpnI SacI SmaI
Polylinker sites: BamHI HindIII XbaI KpnI SphI EcoRV SacI SmaI EcoRI
Vector: pDMS197 (plasmid)
Construction: pRE107, tetR (pBR322)
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
MCS: BamHI...EcoRI, ->
marker(s): sacB1, ->
restriction site: EcoRI HindIII, ->
replicon: conditional R6K ori
replicon: oriT
marker(s): tetR, ->
Applications Counterselection levansucrase
Suicide vector
Vector permitting allelic exchange of cloned inserts
Comments Restriction digests of the clone give the following sizes (kb): XbaI-- 6.55; KpnI-- 6.55; HindIII-- 2.9, 2.2, 1.4.

The sacB1 allele is a variant of sacB with certain restriction sites removed by site directed mutagenesis. Expression of sacB confers sensitivity to sucrose.

The conditional R6K origin of replication requires that the pi protein be expressed in trans for plasmid maintenance.

One of four allelic exchange suicide vectors (ATCC 87691 - 87694) that provide both selection for chromosomal integration (ampR, cmlR, kanR or tetR) and counterselection for loss of vector DNA and the wild type allele.

Construct is suicide plasmid in any host not expressing pir.

Cloned inserts may be integrated into the host chromosome (a single recombination event) following electroporation and appropriate antibiotic selection.

Negative selection for sucrose sensitivity (sacB) selects for a second recombination event resulting in loss of vector DNA.
Media ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline
Growth Conditions
Temperature: 37°C
References

Edwards RA, et al. Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. Gene 207: 149-157, 1998. PubMed: 9511756

Donnenberg MS, Kaper JB. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317, 1991. PubMed: 1937792

Blomfield IC, et al. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5: 1447-1457, 1991. PubMed: 1686293

Kolter R, et al. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15: 1199-1208, 1978. PubMed: 728998

McIver KS, et al. The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa. Mol. Microbiol. 18: 877-889, 1995. PubMed: 8825092

Shipped frozen
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
宾阳县| 西青区| 诸暨市| 东乌珠穆沁旗| 饶平县| 岑溪市| 花莲县| 武山县| 西峡县| 吉水县| 龙海市| 哈巴河县| 吉首市| 阳泉市| 唐河县| 信阳市| 普定县| 措勤县| 清苑县| 灌云县| 屏东市| 剑川县| 墨脱县| 抚顺市| 沂南县| 崇信县| 深州市| 莱州市| 鄂温| 永善县| 临沧市| 门源| 永春县| 云梦县| 咸宁市| 西宁市| 百色市| 隆林| 宾阳县| 荔波县| 全椒县|