| Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
| Component |
| Type Strain |
Volume |
| Biosafety Level |
Composition |
| Product Format |
Storage |
| Storage Conditions |
Lysis Buffer |
| 梅經(jīng)理 |
17280875617 |
1438578920 |
| 胡經(jīng)理 |
13345964880 |
2438244627 |
| 周經(jīng)理 |
17757487661 |
1296385441 |
| 于經(jīng)理 |
18067160830 |
2088210172 |
| 沈經(jīng)理 |
19548299266 |
2662369050 |
| 李經(jīng)理 |
13626845108 |
972239479 |
绥江县|
永仁县|
通渭县|
柘荣县|
门头沟区|
信丰县|
封开县|
兴安县|
绵阳市|
新密市|
酉阳|
江源县|
定安县|
隆林|
甘德县|
武安市|
彰化市|
定州市|
晋宁县|
台湾省|
泸溪县|
开鲁县|
崇义县|
南城县|
区。|
金平|
葫芦岛市|
伊川县|
瓮安县|
蒙山县|
邻水|
大庆市|
高青县|
榆中县|
平陆县|
南丰县|
南陵县|
瑞丽市|
南开区|
广宁县|
康马县|
|
