| 產(chǎn)品名稱(chēng) |
pPD39 |
| 商品貨號(hào) |
B242617 |
| Designations |
pPD39 |
| GenBank Number |
U02043
|
| Species |
Homo sapiens, human |
| Depositors |
PL Deininger |
| Vector |
Construct size (kb): 3.299999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: repetitive
Gene name: Alu consensus sequence, repetitive
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 0.33
Cross references: DNA Seq. Acc.: U02043 Insert lengths(kb): 0.3300000131130219 Gene product: Alu consensus sequence, repetitive |
| Insert Size (kb) |
0.330 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.4; PvuI--2.3, 1.1; SacI--3.4; PvuII--2.6, 0.8; KpnI--3.4. A perfect HS consensus sequence (HSC3N1) modified at the ends (4 bp and 24 bp) for cloning. Hybridizes efficiently to a few Alu sequences in a mouse cell background at 52C in 50% formamide, with a wash at 61C. Because the sequence is closer to consensus than BLUR8, it matches rodent B1 family better with an increase in rodent background. |
| References |
Batzer MA, et al. A consensus Alu repeat probe for physical mapping. GATA 11: 34-38, 1994. PubMed: 7917498
Prescott L Deininger, personal communication
|
