PMA-Free Melanocyte Growth Supplement (MelGS-2) is a medium supplement designed for the optimal growth of normal human melanocytes in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human melanocytes. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human melanocytes in vitro. The supplement is designed as an additive for melanocyte medium (MelM-2, cat. no. 2211) and should be used in conjunction with that medium.