| 備注 |
培養(yǎng)基配方:Trypticasepeptone5.0gPeptonefrommeat(pepsin-digested)5.0gYeastextract10.0gNa-resazurin0.0005gL-Cysteine-HCl.H2O0.5gCaCl2.2H2O0.01gMgSO4.7H2O0.02gK2HPO40.04gKH2PO40.04gNaHCO30.4gNaCl0.08g；使用說明:Dissolveingredients(21.09g/L)andspargemediumwith100%N2gasfor30–45mintomakeitanoxic.AddcysteineandadjustpHto7.0,thendispensemediumunder100%N2gasatmosphereintoanoxicHungate-typetubesorserumvialsandautoclave.AdjustpHofcompletemediumto6.8-7.0,ifnecessary. |
